| |
|
|
| Registros recuperados : 2 | |
| 1. |  | BUFFO, M. M.; FERREIRA, A. L. Z.; ALMEIDA, R. M. R. G.; BADINO, A. C.; FARINAS, C. S. Mudança da morfologia de A. Niger: aplicação na produção de enzimas celulolíticas. In: SIMPÓSIO NACIONAL DE INSTRUMENTAÇÃO AGROPECUÁRIA, 4., 2019, São Carlos, SP. Ciência, inovação e mercado: anais. São Carlos, SP: Embrapa Instrumentação, 2019. Editores: Paulino Ribeiro Villas-Boas, Maria Alice Martins, Débora Marcondes Bastos Pereira Milori, Ladislau Martin Neto. SIAGRO 2019. 284-288| Biblioteca(s): Embrapa Instrumentação. |
|    |
| 2. | | BUFFO, M. M.; FERREIRA, A. L. Z.; ALMEIDA, R. M. R. G.; FARINAS, C. S.; BADINO, A. C.; XIMENES, E. A.; LADISCH, M. R. Cellulolytic enzymes production guided by morphology engineering. Enzyme and Microbial Technology, v. 149, e109833, 2021. 1-8| Biblioteca(s): Embrapa Instrumentação. |
|  |
| Registros recuperados : 2 | |
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Instrumentação. |
|
Data corrente: |
23/06/2021 |
|
Data da última atualização: |
10/06/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
BUFFO, M. M.; FERREIRA, A. L. Z.; ALMEIDA, R. M. R. G.; FARINAS, C. S.; BADINO, A. C.; XIMENES, E. A.; LADISCH, M. R. |
|
Afiliação: |
CRISTIANE SANCHEZ FARINAS, CNPDIA. |
|
Título: |
Cellulolytic enzymes production guided by morphology engineering. |
|
Ano de publicação: |
2021 |
|
Fonte/Imprenta: |
Enzyme and Microbial Technology, v. 149, e109833, 2021. |
|
Páginas: |
1-8 |
|
ISSN: |
0141-0229 |
|
DOI: |
https://doi.org/10.1016/j.enzmictec.2021.109833 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Endoglucanase and xylanase are critical enzymes for liquefaction and enzyme hydrolysis of high solids lignocellulosic biomass to facilitate its transport and production of desired derived products. Here is reported how combinations of different spore concentrations and pH influence microbial morphology, and how this may be used to direct expression and secretion of enzymes by Aspergillus niger. While xylanase production is not affected by A. niger morphology changes, endoglucanase production is enhanced under conditions of lower stress and by morphology that results in pellets. β-glucosidase production is enhanced under dispersed morphology, which results in up to fourfold increase of this enzyme production under the tested experimental conditions. A morphologic scale (Y) is proposed based on a form factor that considers the size and frequency of each morphology class, and that points to conditions that result in high selectivity for either endoglucanase or β-glucosidase production. An equation proposed to relate enzyme activity to morphology provides a useful tool for tuning enzyme production of A. niger, where morphology is a first indication of relative enzyme activities in a fermentation broth. |
|
Palavras-Chave: |
(hemi) Cellulases; Filamentous fungi; Morphology engineering. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02002naa a2200265 a 4500
001 2132521
005 2022-06-10
008 2021 bl uuuu u00u1 u #d
022 $a0141-0229
024 7 $ahttps://doi.org/10.1016/j.enzmictec.2021.109833$2DOI
100 1 $aBUFFO, M. M.
245 $aCellulolytic enzymes production guided by morphology engineering.$h[electronic resource]
260 $c2021
300 $a1-8
520 $aEndoglucanase and xylanase are critical enzymes for liquefaction and enzyme hydrolysis of high solids lignocellulosic biomass to facilitate its transport and production of desired derived products. Here is reported how combinations of different spore concentrations and pH influence microbial morphology, and how this may be used to direct expression and secretion of enzymes by Aspergillus niger. While xylanase production is not affected by A. niger morphology changes, endoglucanase production is enhanced under conditions of lower stress and by morphology that results in pellets. β-glucosidase production is enhanced under dispersed morphology, which results in up to fourfold increase of this enzyme production under the tested experimental conditions. A morphologic scale (Y) is proposed based on a form factor that considers the size and frequency of each morphology class, and that points to conditions that result in high selectivity for either endoglucanase or β-glucosidase production. An equation proposed to relate enzyme activity to morphology provides a useful tool for tuning enzyme production of A. niger, where morphology is a first indication of relative enzyme activities in a fermentation broth.
653 $a(hemi) Cellulases
653 $aFilamentous fungi
653 $aMorphology engineering
700 1 $aFERREIRA, A. L. Z.
700 1 $aALMEIDA, R. M. R. G.
700 1 $aFARINAS, C. S.
700 1 $aBADINO, A. C.
700 1 $aXIMENES, E. A.
700 1 $aLADISCH, M. R.
773 $tEnzyme and Microbial Technology$gv. 149, e109833, 2021.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Instrumentação (CNPDIA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
